How to Reconstitute Peptides: A Complete Guide
Reconstitution is the process of adding bacteriostatic water (BAC water) to a lyophilized (freeze-dried) peptide powder to create an injectable solution. Proper reconstitution is critical for accurate dosing and peptide stability.
Step 1: Gather Your Supplies
You will need: your peptide vial, bacteriostatic water, an insulin syringe (typically U-100), and alcohol swabs. Ensure everything is sterile and your workspace is clean.
Step 2: Determine the Water Volume
The amount of bacteriostatic water you add determines the concentration. More water = lower concentration = larger injection volumes. Less water = higher concentration = smaller volumes. Use the calculator above to find the optimal balance.
Step 3: Add Water to the Vial
Draw the desired amount of BAC water into your syringe. Insert the needle through the rubber stopper and aim the stream along the glass wall of the vial — do not spray directly onto the powder, as this can damage the peptide. Let the water gently roll down the side.
Step 4: Mix Gently
After adding water, gently swirl the vial. Never shake a peptide vial — shaking creates bubbles and can denature the peptide. Some peptides dissolve quickly; others may need a few minutes. If needed, roll the vial between your palms.
Step 5: Calculate and Draw Your Dose
Once reconstituted, use the concentration to determine how much volume to draw for your desired dose. Our calculator shows this in both mL and insulin units (IU) for U-100 syringes, along with a visual syringe guide.
Storage After Reconstitution
Reconstituted peptides must be stored in the refrigerator (2–8°C / 36–46°F). Most reconstituted peptides remain stable for 3–4 weeks when properly stored. Never freeze a reconstituted solution, and always use sterile technique when drawing doses.
Understanding Insulin Syringe Units
U-100 insulin syringes are calibrated so that 100 units equals 1 mL. This means 10 units = 0.1 mL, 50 units = 0.5 mL, and so on. The smaller markings on the syringe typically represent 1 or 2 units each. Our visual syringe guide shows exactly where to draw to for your calculated dose.